4JX1
Crystal structure of reduced Cytochrome P450cam-putidaredoxin complex bound to camphor and 5-exo-hydroxycamphor
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, E, F (A, B, E, F) | Camphor 5-monooxygenase | polymer | 415 | 46597.7 | 4 | UniProt (P00183) | Pseudomonas putida | Cytochrome P450-cam, Cytochrome P450cam |
| 2 | C, D, G, H (C, D, G, H) | Putidaredoxin | polymer | 113 | 12361.0 | 4 | UniProt (P00259) | Pseudomonas putida | PDX |
| 3 | I, M, R, W (A, B, E, F) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 4 | Chemie (HEM) | |||
| 4 | J, N, S, X (A, B, E, F) | CAMPHOR | non-polymer | 152.2 | 4 | Chemie (CAM) PubChem (2537) PubChem (159055) PubChem (230921) PubChem (444294) PubChem (5284500) PubChem (6857773) PubChem (9543187) PubChem (23308299) PubChem (10050) | |||
| 5 | K, T, Y (A, E, F) | 5-EXO-HYDROXYCAMPHOR | non-polymer | 168.2 | 3 | Chemie (CAH) PubChem (440017) PubChem (447414) PubChem (5459815) PubChem (9543191) PubChem (14635301) PubChem (20057218) PubChem (21145050) PubChem (49874362) PubChem (55277468) PubChem (98457732) PubChem (123773823) PubChem (130813327) | |||
| 6 | L, O, V, Z (A, B, E, F) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) PubChem (271) | |||
| 7 | AA, BA, P, Q (G, H, C, D) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 4 | Chemie (FES) | |||
| 8 | U (E) | 1,1'-hexane-1,6-diyldipyrrolidine-2,5-dione | non-polymer | 280.3 | 1 | Chemie (1N0) PubChem (341888) | |||
| 9 | CA, DA, EA, FA, GA... (A, B, C, D, E...) | water | water | 18.0 | 1948 | Chemie (HOH) |
Sequence modifications
A, B, E, F: 0 - 414 (UniProt: P00183)
C, D, G, H: 0 - 106 (UniProt: P00259)
| PDB | External Database | Details |
|---|---|---|
| Ser 58 | Cys 59 | engineered mutation |
| Ser 85 | Cys 86 | engineered mutation |
| Ser 136 | Cys 137 | engineered mutation |
| Ser 285 | Cys 286 | engineered mutation |
| Ala 334 | Cys 335 | engineered mutation |
| Cys 344 | Lys 345 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| Cys 19 | Asp 20 | engineered mutation |
| Ser 73 | Cys 74 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 235834.8 | |
| Non-Polymers* | Number of molecules | 20 |
| Total formula weight | 4723.5 | |
| All* | Total formula weight | 240558.3 |
