4JX1

Crystal structure of reduced Cytochrome P450cam-putidaredoxin complex bound to camphor and 5-exo-hydroxycamphor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (A, B, E, F)	Camphor 5-monooxygenase	polymer	415	46597.7	4	UniProt (P00183) Pfam (PF00067)	Pseudomonas putida	Cytochrome P450-cam, Cytochrome P450cam
2	C, D, G, H (C, D, G, H)	Putidaredoxin	polymer	113	12361.0	4	UniProt (P00259) Pfam (PF00111)	Pseudomonas putida	PDX
3	I, M, R, W (A, B, E, F)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	4	Chemie (HEM)
4	J, N, S, X (A, B, E, F)	CAMPHOR	non-polymer		152.2	4	Chemie (CAM)
5	K, T, Y (A, E, F)	5-EXO-HYDROXYCAMPHOR	non-polymer		168.2	3	Chemie (CAH)
6	L, O, V, Z (A, B, E, F)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
7	AA, BA, P, Q (G, H, C, D)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	4	Chemie (FES)
8	U (E)	1,1'-hexane-1,6-diyldipyrrolidine-2,5-dione	non-polymer		280.3	1	Chemie (1N0)
9	CA, DA, EA, FA, GA... (A, B, C, D, E...)	water	water		18.0	1948	Chemie (HOH)

Sequence modifications

A, B, E, F: 0 - 414 (UniProt: P00183)

PDB	External Database	Details
Ser 58	Cys 59	engineered mutation
Ser 85	Cys 86	engineered mutation
Ser 136	Cys 137	engineered mutation
Ser 285	Cys 286	engineered mutation
Ala 334	Cys 335	engineered mutation
Cys 344	Lys 345	engineered mutation

C, D, G, H: 0 - 106 (UniProt: P00259)

PDB	External Database	Details
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Cys 19	Asp 20	engineered mutation
Ser 73	Cys 74	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	235834.8
Non-Polymers*	Number of molecules	20
	Total formula weight	4723.5
All*	Total formula weight	240558.3

*Water molecules are not included.