4JX1
Crystal structure of reduced Cytochrome P450cam-putidaredoxin complex bound to camphor and 5-exo-hydroxycamphor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.09 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.850, 108.960, 115.160 |
| Unit cell angles | 90.00, 109.03, 90.00 |
Refinement procedure
| Resolution | 44.870 - 2.087 |
| R-factor | 0.1472 |
| Rwork | 0.144 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2CPP AND 1XLO |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.519 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.200 | 2.200 |
| High resolution limit [Å] | 2.087 | 2.087 |
| Rmerge | 0.065 | 0.159 |
| Number of reflections | 118774 | |
| <I/σ(I)> | 15.3 | 7.9 |
| Completeness [%] | 98.8 | 98.3 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.2 M calcium acetate hydrate, 14-22% PEG3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
