4JK0

Crystal structure of T89Q-mutant of RNA silencing suppressor p19 with 2nt-5'-overhanging double-helical RNA 21mer pUUUG(CUG)5CU

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (D)	RNA silencing suppressor p19	polymer	127	14540.2	1	UniProt (P69517) Pfam (PF03220)	Tomato bushy stunt virus (TBSV)	19 kDa symptom severity modulator
2	B (B)	5'-R(P*UP*UP*UP*GP*CP*UP*GP*CP*UP*GP*CP*UP*GP*CP*UP*GP*CP*UP*GP*CP*U)-3'	polymer	21	6612.9	1
3	C, D (B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	E, F (D, B)	water	water		18.0	187	Chemie (HOH)

Sequence modifications

D: 5 - 127 (UniProt: P69517)

PDB	External Database	Details
Gly 1	-	expression tag
Ser 2	-	expression tag
His 3	-	expression tag
Met 4	-	expression tag
Gln 89	Thr 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	21153.1
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	21345.2

*Water molecules are not included.