4JK0

Crystal structure of T89Q-mutant of RNA silencing suppressor p19 with 2nt-5'-overhanging double-helical RNA 21mer pUUUG(CUG)5CU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9724
Spacegroup name	H 3 2
Unit cell lengths	90.391, 90.391, 148.909
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.1867
Rwork	0.184
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r9f
RMSD bond length	0.013
RMSD bond angle	1.550
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.118	0.064	0.630
Number of reflections	10511
<I/σ(I)>	9.1
Completeness [%]	98.6	94.6	98.5
Redundancy	2.7	2.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	1.6 M ammonium sulfate, 0.1 M citric acid, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 291K