4JJJ
The structure of T. fusca GH48 D224N mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Cellulose 1,4-beta-cellobiosidase | polymer | 642 | 71759.3 | 1 | UniProt (Q47NH7) Pfam (PF02011) In PDB | Thermobifida fusca | |
| 2 | B | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900005) GlyTouCan (G84824ZO) | beta-cellobiose | ||
| 3 | C | beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900023) GlyTouCan (G86802XL) | alpha-cellobiose | ||
| 4 | D | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 990.9 | 1 | In PDB BIRD (PRD_900020) GlyTouCan (G09454VW) | beta-cellohexaose | ||
| 5 | A | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 6 | A | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
| 7 | A | FE (III) ION | non-polymer | 55.8 | 4 | Chemie (FE) | |||
| 8 | A | ZINC ION | non-polymer | 65.4 | 21 | Chemie (ZN) | |||
| 9 | A | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 10 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 11 | water | water | 18.0 | 1136 | Chemie (HOH) |
Sequence modifications
A: 1 - 642 (UniProt: Q47NH7)
| PDB | External Database | Details |
|---|---|---|
| Asn 224 | Asp 566 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 71759.3 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1675.5 | |
| Non-Polymers* | Number of molecules | 35 |
| Total formula weight | 2006.4 | |
| All* | Total formula weight | 75441.2 |
