4JJJ
The structure of T. fusca GH48 D224N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-30 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.458, 88.494, 66.753 |
| Unit cell angles | 90.00, 113.72, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.600 |
| R-factor | 0.12351 |
| Rwork | 0.121 |
| R-free | 0.16379 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fus |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.152 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 78727 | |
| <I/σ(I)> | 15.1 | 3.81 |
| Completeness [%] | 99.4 | 93.9 |
| Redundancy | 7.93 | 3.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.1 M sodium cacodylate trihydrate pH 6.5, 18% (w/v) PEG 8000 and 20 mM zinc acetate dihydrate, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
