4JIO

Bro1 V domain and ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	BRO1	polymer	344	39612.8	2	UniProt (G0VBG6) Pfam (PF13949)	Naumovozyma castellii (Yeast)
2	C (U)	Ubiquitin	polymer	76	8636.9	1	UniProt (P62987) Pfam (PF00240)	Homo sapiens (human)

Sequence modifications

A, B: 370 - 708 (UniProt: G0VBG6)

PDB	External Database	Details
Ile 365	-	expression tag
Asp 366	-	expression tag
Pro 367	-	expression tag
Phe 368	-	expression tag
Thr 369	-	expression tag
Ala 417	Lys 417	engineered mutation
Ala 418	Lys 418	engineered mutation
Ala 419	Lys 419	engineered mutation

U: 1 - 76 (UniProt: P62987)

PDB	External Database	Details
Met 28	Ala 28	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	87862.5
All*	Total formula weight	87862.5

*Water molecules are not included.