4JHD

Crystal Structure of an Actin Dimer in Complex with the Actin Nucleator Cordon-Bleu

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Actin-5C	polymer	384	42989.0	2	UniProt (P10987) Pfam (PF00022)	Drosophila melanogaster (Fruit fly)
2	B, E (B, E)	Actin-5C	polymer	384	42930.9	2	UniProt (P10987) Pfam (PF00022)	Drosophila melanogaster (Fruit fly)
3	C, F (C, F)	Protein cordon-bleu	polymer	171	18136.6	2	UniProt (Q5NBX1) Pfam (PF02205)	Mus musculus (mouse)
4	G, I, K, M (A, B, D, E)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	4	Chemie (ANP)
5	H, J, L, N (A, B, D, E)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
6	O, P, Q, R, S... (A, B, C, D, E...)	water	water		18.0	158	Chemie (HOH)

Sequence modifications

A, D: 0 - 375 (UniProt: P10987)

PDB	External Database	Details
Met -8	-	expression tag
Ala -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Glu 204	Ala 205	engineered mutation
Lys 243	Pro 244	engineered mutation

B, E: 0 - 375 (UniProt: P10987)

PDB	External Database	Details
Met -8	-	expression tag
Ala -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Glu 291	Lys 292	engineered mutation
Lys 322	Pro 323	engineered mutation

C, F: 58 - 219 (UniProt: Q5NBX1)

PDB	External Database	Details
Met 49	-	expression tag
Ala 50	-	expression tag
His 51	-	expression tag
His 52	-	expression tag
His 53	-	expression tag
His 54	-	expression tag
His 55	-	expression tag
His 56	-	expression tag
Val 57	-	expression tag
Val 87	Thr 1205	SEE REMARK 999
Thr 167	Pro 1285	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	208113.0
Non-Polymers*	Number of molecules	8
	Total formula weight	2122.0
All*	Total formula weight	210235.0

*Water molecules are not included.