4JB4

Expression, Purification, Characterization, and Solution NMR Study of Highly Deuterated Yeast Cytochrome c Peroxidase with Enhanced Solubility

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Cytochrome c peroxidase, mitochondrial	polymer	300	34462.3	2	UniProt (P00431) Pfam (PF00141) In PDB	Saccharomyces cerevisiae (Baker's yeast)	CCP
2	A, C	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
3	A, C	FLUORIDE ION	non-polymer		19.0	2	Chemie (F)
4		water	water		18.0	36	Chemie (HOH)

Sequence modifications

A, C: 1 - 294 (UniProt: P00431)

PDB	External Database	Details
Met 1	Thr 68	engineered mutation
Lys 2	Thr 69	engineered mutation
Thr 3	Pro 70	engineered mutation
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	68924.5
Non-Polymers*	Number of molecules	4
	Total formula weight	1271.0
All*	Total formula weight	70195.5

*Water molecules are not included.