4JB4
Expression, Purification, Characterization, and Solution NMR Study of Highly Deuterated Yeast Cytochrome c Peroxidase with Enhanced Solubility
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, C) | Cytochrome c peroxidase, mitochondrial | polymer | 300 | 34462.3 | 2 | UniProt (P00431) Pfam (PF00141) | Saccharomyces cerevisiae (Baker's yeast) | CCP |
| 2 | C, E (A, C) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
| 3 | D, F (A, C) | FLUORIDE ION | non-polymer | 19.0 | 2 | Chemie (F) | |||
| 4 | G, H (A, C) | water | water | 18.0 | 36 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 294 (UniProt: P00431)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | Thr 68 | engineered mutation |
| Lys 2 | Thr 69 | engineered mutation |
| Thr 3 | Pro 70 | engineered mutation |
| His 295 | - | expression tag |
| His 296 | - | expression tag |
| His 297 | - | expression tag |
| His 298 | - | expression tag |
| His 299 | - | expression tag |
| His 300 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 68924.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1271.0 | |
| All* | Total formula weight | 70195.5 |
