4JB4
Expression, Purification, Characterization, and Solution NMR Study of Highly Deuterated Yeast Cytochrome c Peroxidase with Enhanced Solubility
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-05-04 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.052, 87.920, 87.526 |
| Unit cell angles | 90.00, 105.14, 90.00 |
Refinement procedure
| Resolution | 43.570 - 2.390 |
| R-factor | 0.2156 |
| Rwork | 0.212 |
| R-free | 0.27937 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zby |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.663 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.450 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.158 | |
| Completeness [%] | 99.0 | 90.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.2M NH4Cl, 0.1 M CH3COONa pH5.0 and 20% w/v PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
