4JAV
Structural basis of a rationally rewired protein-protein interface (HK853wt and RR468mutant V13P, L14I, I17M and N21V)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Histidine kinase | polymer | 258 | 29378.3 | 2 | UniProt (Q9WZV7) Pfam (PF00512) Pfam (PF02518) | Thermotoga maritima | Sensor histidine kinase |
| 2 | C, D (C, D) | Response regulator | polymer | 122 | 13914.3 | 2 | UniProt (Q9WYT9) Pfam (PF00072) | Thermotoga maritima | RR468, Response regulator receiver protein |
| 3 | E, F, I, J, K (A, B) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 4 | G, L, O, P (A, B, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 5 | H, M (A, B) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) | |||
| 6 | N (B) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 7 | Q, R, S (A, B, C) | water | water | 18.0 | 13 | Chemie (HOH) |
Sequence modifications
C, D: 1 - 122 (UniProt: Q9WYT9)
| PDB | External Database | Details |
|---|---|---|
| Pro 13 | Val 13 | engineered mutation |
| Ile 14 | Leu 14 | engineered mutation |
| Met 17 | Ile 17 | engineered mutation |
| Val 21 | Asn 21 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 86585.2 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 1467.4 | |
| All* | Total formula weight | 88052.6 |
