4JAL

Crystal structure of tRNA (Um34/Cm34) methyltransferase TrmL from Escherichia coli with SAH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	tRNA (cytidine(34)-2'-O)-methyltransferase	polymer	167	18832.4	2	UniProt (P0AGJ7) Pfam (PF00588)	Escherichia coli	tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmL
2	C, G (A, B)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
3	D (A)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
4	E, F (B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5	H, I (A, B)	water	water		18.0	48	Chemie (HOH)

Sequence modifications

A, B: 2 - 157 (UniProt: P0AGJ7)

PDB	External Database	Details
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
Met 0	-	expression tag
Ala 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	37664.7
Non-Polymers*	Number of molecules	5
	Total formula weight	1131.3
All*	Total formula weight	38796.0

*Water molecules are not included.