4JAL

Crystal structure of tRNA (Um34/Cm34) methyltransferase TrmL from Escherichia coli with SAH

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001510	biological_process	RNA methylation
A	0002131	biological_process	wobble position cytosine ribose methylation
A	0002132	biological_process	wobble position uridine ribose methylation
A	0003723	molecular_function	RNA binding
A	0005737	cellular_component	cytoplasm
A	0006396	biological_process	RNA processing
A	0008033	biological_process	tRNA processing
A	0008168	molecular_function	methyltransferase activity
A	0008173	molecular_function	RNA methyltransferase activity
A	0008175	molecular_function	tRNA methyltransferase activity
A	0008757	molecular_function	S-adenosylmethionine-dependent methyltransferase activity
A	0016740	molecular_function	transferase activity
A	0030488	biological_process	tRNA methylation
A	0032259	biological_process	methylation
A	0042803	molecular_function	protein homodimerization activity
A	0052665	molecular_function	tRNA (uracil-2'-O-)-methyltransferase activity
A	0141098	molecular_function	tRNA (cytidine(34)-2'-O)-methyltransferase activity
A	0141102	molecular_function	tRNA (5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase activity
B	0001510	biological_process	RNA methylation
B	0002131	biological_process	wobble position cytosine ribose methylation
B	0002132	biological_process	wobble position uridine ribose methylation
B	0003723	molecular_function	RNA binding
B	0005737	cellular_component	cytoplasm
B	0006396	biological_process	RNA processing
B	0008033	biological_process	tRNA processing
B	0008168	molecular_function	methyltransferase activity
B	0008173	molecular_function	RNA methyltransferase activity
B	0008175	molecular_function	tRNA methyltransferase activity
B	0008757	molecular_function	S-adenosylmethionine-dependent methyltransferase activity
B	0016740	molecular_function	transferase activity
B	0030488	biological_process	tRNA methylation
B	0032259	biological_process	methylation
B	0042803	molecular_function	protein homodimerization activity
B	0052665	molecular_function	tRNA (uracil-2'-O-)-methyltransferase activity
B	0141098	molecular_function	tRNA (cytidine(34)-2'-O)-methyltransferase activity
B	0141102	molecular_function	tRNA (5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	17
Details	BINDING SITE FOR RESIDUE SAH A 201

Chain	Residue
A	ASN14
A	ILE122
A	MET124
A	SER130
A	MET131
A	ASN132
A	LEU133
A	EPE202
A	HOH303
A	LEU78
A	THR79
A	PHE99
A	GLY100
A	PRO101
A	GLU102
A	GLY105
A	ILE120

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site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EPE A 202

Chain	Residue
A	THR79
A	THR80
A	LYS81
A	THR103
A	GLY105
A	LEU106
A	SAH201

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site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO B 201

Chain	Residue
A	ASN17
A	ARG20
A	LEU21
B	MET131
B	ASN132
B	ASN135
B	HOH310

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site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO B 202

Chain	Residue
B	LEU78
B	THR79
B	THR80
B	LEU106
B	SAH203

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site_id	AC5
Number of Residues	15
Details	BINDING SITE FOR RESIDUE SAH B 203

Chain	Residue
A	ALA116
A	GLU117
B	LEU78
B	THR79
B	PHE99
B	GLY100
B	PRO101
B	GLU102
B	GLY105
B	ILE120
B	ILE122
B	MET124
B	SER130
B	MET131
B	EDO202

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01885

Chain	Residue	Details
A	LEU78
A	GLY100
A	ILE122
A	SER130
B	LEU78
B	GLY100
B	ILE122
B	SER130

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