4J8A
Irradiated-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 145
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Green fluorescent protein | polymer | 247 | 27886.4 | 1 | UniProt (P42212) Pfam (PF01353) In PDB | Aequorea victoria (Jellyfish) | |
2 | A | SULFATE ION | non-polymer | 96.1 | 6 | Chemie (SO4) | |||
3 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 30 | Chemie (EDO) | |||
4 | A | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
5 | water | water | 18.0 | 260 | Chemie (HOH) |
Sequence modifications
A: 2 - 238 (UniProt: P42212)
PDB | External Database | Details |
---|---|---|
Met 0 | - | expression tag |
Val 1 | - | expression tag |
Arg 30 | Ser 30 | engineered mutation |
Asn 39 | Tyr 39 | engineered mutation |
Cro 66 | Phe 64 | SEE REMARK 999 |
Cro 66 | Ser 65 | SEE REMARK 999 |
Cro 66 | Tyr 66 | SEE REMARK 999 |
Cro 66 | Gly 67 | SEE REMARK 999 |
Arg 80 | Gln 80 | engineered mutation |
Ser 99 | Phe 99 | engineered mutation |
Thr 105 | Asn 105 | engineered mutation |
Hox 145 | Tyr 145 | engineered mutation |
Thr 153 | Met 153 | engineered mutation |
Ala 163 | Val 163 | engineered mutation |
Val 171 | Ile 171 | engineered mutation |
Val 206 | Ala 206 | engineered mutation |
Gly 239 | - | expression tag |
Ser 240 | - | expression tag |
His 241 | - | expression tag |
His 242 | - | expression tag |
His 243 | - | expression tag |
His 244 | - | expression tag |
His 245 | - | expression tag |
His 246 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 27886.4 | |
Non-Polymers* | Number of molecules | 37 |
Total formula weight | 2560.6 | |
All* | Total formula weight | 30446.9 |