4J8A
Irradiated-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 145
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-19 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.500, 58.700, 91.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.790 - 1.260 |
R-factor | 0.1305 |
Rwork | 0.129 |
R-free | 0.16460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
RMSD bond length | 0.023 |
RMSD bond angle | 1.586 |
Data reduction software | xia2 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.500 |
High resolution limit [Å] | 1.260 |
Rmerge | 0.032 |
Number of reflections | 67853 |
<I/σ(I)> | 16 |
Completeness [%] | 99.2 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 278 | 10 mg/mL protein and 100 mM Tris-HCl, pH 8.3, 2 M (NH4)2SO4, 200+200 nanoL drop against 60 microL reservoir, VAPOR DIFFUSION, SITTING DROP, temperature 278K |