4J88

Dark-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 66

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Green fluorescent protein	polymer	245	27896.3	2	UniProt (P42212) Pfam (PF01353)	Aequorea victoria (Jellyfish)
2	C, CA, D, DA, E... (A, B)	SULFATE ION	non-polymer		96.1	9	Chemie (SO4)
3	G, H, HA, I, IA... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	24	Chemie (EDO)
4	AA, BA, Z (A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	3	Chemie (TRS)
5	MA, NA (A, B)	water	water		18.0	314	Chemie (HOH)

Sequence modifications

A, B: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Met 0	-	expression tag
Val 1	-	expression tag
Arg 30	Ser 30	engineered mutation
Asn 39	Tyr 39	engineered mutation
Cq1 66	Phe 64	SEE REMARK 999
Cq1 66	Ser 65	SEE REMARK 999
Leu 64	Tyr 66	SEE REMARK 999
Cq1 66	Gly 67	SEE REMARK 999
Arg 80	Gln 80	engineered mutation
Ser 99	Phe 99	engineered mutation
Thr 105	Asn 105	engineered mutation
Phe 145	Tyr 145	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Val 171	Ile 171	engineered mutation
Val 206	Ala 206	engineered mutation
Gly 239	-	expression tag
Ser 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag
His 245	-	expression tag
His 246	-	expression tag
Cq1 66	Phe 64	SEE REMARK 999
Cq1 66	Ser 65	SEE REMARK 999
Leu 64	Tyr 66	SEE REMARK 999
Cq1 66	Gly 67	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55792.7
Non-Polymers*	Number of molecules	36
	Total formula weight	2720.6
All*	Total formula weight	58513.3

*Water molecules are not included.