4IZU

The E41Q mutant of the amidase from Nesterenkonia sp. AN1 showing the result of Michael addition of acrylamide at the active site cysteine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Amidase	polymer	283	30099.8	1	UniProt (D0VWZ1) Pfam (PF00795) In PDB	Nesterenkonia sp. 10004
2	A	PROPIONAMIDE	non-polymer		73.1	2	Chemie (ROP)
3	A	prop-2-enamide	non-polymer		71.1	1	Chemie (1HC)
4		water	water		18.0	313	Chemie (HOH)

Sequence modifications

A: 1 - 263 (UniProt: D0VWZ1)

PDB	External Database	Details
Met -19	-	INITIATING METHIONINE
Gly -18	-	EXPRESSION TAG
Ser -17	-	EXPRESSION TAG
Ser -16	-	EXPRESSION TAG
His -15	-	EXPRESSION TAG
His -14	-	EXPRESSION TAG
His -13	-	EXPRESSION TAG
His -12	-	EXPRESSION TAG
His -11	-	EXPRESSION TAG
His -10	-	EXPRESSION TAG
Ser -9	-	EXPRESSION TAG
Ser -8	-	EXPRESSION TAG
Gly -7	-	EXPRESSION TAG
Leu -6	-	EXPRESSION TAG
Val -5	-	EXPRESSION TAG
Pro -4	-	EXPRESSION TAG
Arg -3	-	EXPRESSION TAG
Gly -2	-	EXPRESSION TAG
Ser -1	-	EXPRESSION TAG
His 0	-	EXPRESSION TAG
Gln 41	Glu 41	ENGINEERED MUTATION

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30099.8
Non-Polymers*	Number of molecules	3
	Total formula weight	217.3
All*	Total formula weight	30317.0

*Water molecules are not included.