4IZU
The E41Q mutant of the amidase from Nesterenkonia sp. AN1 showing the result of Michael addition of acrylamide at the active site cysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-10 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.102, 115.452, 65.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.050 - 1.400 |
| R-factor | 0.174 |
| Rwork | 0.173 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.481 |
| Data reduction software | d*TREK (9.7L) |
| Data scaling software | d*TREK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.050 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.066 | 0.384 |
| Number of reflections | 57302 | |
| <I/σ(I)> | 9.8 | 3.2 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 298 | 0.2 M ammonium tartrate dibasic, 20% PEG 3350, vapor diffusion, sitting drop, temperature 298K |
