4IZN

Structure of pcDronpa-A69T mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Fluorescent protein Dronpa	polymer	257	29180.9	12	UniProt (Q5TLG6) Pfam (PF01353) In PDB	Echinophyllia
2	A, B, C, D, E...	POTASSIUM ION	non-polymer		39.1	12	Chemie (K)
3		water	water		18.0	1481	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L: 1 - 224 (UniProt: Q5TLG6)

PDB	External Database	Details
Met -32	-	expression tag
Arg -31	-	expression tag
Gly -30	-	expression tag
Ser -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
Gly -22	-	expression tag
Met -21	-	expression tag
Ala -20	-	expression tag
Ser -19	-	expression tag
Met -18	-	expression tag
Thr -17	-	expression tag
Gly -16	-	expression tag
Gly -15	-	expression tag
Gln -14	-	expression tag
Gln -13	-	expression tag
Met -12	-	expression tag
Gly -11	-	expression tag
Arg -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Ala 60	Val 60	engineered mutation
Cr8 64	Cys 62	chromophore
Cr8 64	Tyr 63	chromophore
Cr8 64	Gly 64	chromophore
Thr 69	Ala 69	engineered mutation
Ser 94	Asn 94	engineered mutation
Ile 102	Asn 102	engineered mutation
Gly 218	Glu 218	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	350170.9
Non-Polymers*	Number of molecules	12
	Total formula weight	469.2
All*	Total formula weight	350640.1

*Water molecules are not included.