4IZN
Structure of pcDronpa-A69T mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.700, 180.930, 113.161 |
| Unit cell angles | 90.00, 93.37, 90.00 |
Refinement procedure
| Resolution | 34.803 - 2.150 |
| R-factor | 0.1949 |
| Rwork | 0.192 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ie2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.158 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.803 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.769 | |
| Number of reflections | 161957 | |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 0.2M KCl, 0.05M HEPES, 32%(v/v) PEP 426, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
