4IZJ

Crystal structure of yellowtail ascites virus VP4 protease with a wild-type active site reveals acyl-enzyme complexes and product complexes.

Replaces:  3R0B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Yellowtail Ascites Virus (YAV) VP4 protease	polymer	210	22344.4	2	UniProt (P89521) Pfam (PF01768) In PDB	Yellowtail ascites virus
2	B, C	Yellowtail Ascites Virus (YAV) VP4 protease	polymer	210	22328.4	2	UniProt (P89521) Pfam (PF01768) In PDB	Yellowtail ascites virus
3	D	Yellowtail Ascites Virus (YAV) VP4 protease	polymer	210	22328.4	1	UniProt (P89521) Pfam (PF01768) In PDB	Yellowtail ascites virus
4	A, B, C, D, E	BETA-MERCAPTOETHANOL	non-polymer		78.1	6	Chemie (BME)
5	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	D	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
7		water	water		18.0	271	Chemie (HOH)

Sequence modifications

A, E: 508 - 716 (UniProt: P89521)

PDB	External Database	Details
Met 507	-	initiating methionine
Asp 616	Asn 616	engineered mutation

B, C: 508 - 716 (UniProt: P89521)

PDB	External Database	Details
Met 507	-	initiating methionine
Asp 616	Asn 616	engineered mutation

D: 508 - 716 (UniProt: P89521)

PDB	External Database	Details
Met 507	-	initiating methionine
Asp 616	Asn 616	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	111674.1
Non-Polymers*	Number of molecules	8
	Total formula weight	585.2
All*	Total formula weight	112259.3

*Water molecules are not included.