4IRD

Structure of Polymerase-DNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (G, B)	DNA (5'-D(*TP*CP*TP*TP*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3')	polymer	18	5483.5	2
2	B (H)	DNA (5'-D(*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3')	polymer	14	4281.8	1
3	D (C)	DNA (5'-D(*CP*TP*AP*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3')	polymer	17	5188.4	1
4	E, F (F, A)	DNA polymerase IV	polymer	342	38304.3	2	UniProt (Q47155) Pfam (PF00817) Pfam (PF21999) Pfam (PF11799)	Escherichia coli	Pol IV
5	G, J (F, A)	2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]adenosine	non-polymer		490.2	2	Chemie (DZ4)
6	H, I, K, L (F, A)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
7	M, N, O, P, Q... (G, H, B, C, F...)	water	water		18.0	106	Chemie (HOH)

Sequence modifications

F, A: 2 - 341 (UniProt: Q47155)

PDB	External Database	Details
Gly 0	-	expression tag
Ser 1	-	expression tag
Ala 64	Lys 64	conflict
Ala 205	Lys 205	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	97045.8
Non-Polymers*	Number of molecules	6
	Total formula weight	1077.6
All*	Total formula weight	98123.4

*Water molecules are not included.