4IQJ

Structure of PolIIIalpha-Tauc-DNA complex suggests an atomic model of the replisome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E, G, K	DNA (5'-D(P*CP*GP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*A)-3')	polymer	20	6138.0	3		Synthetic construct
2	F, H, J, L	DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*GP*TP*GP*GP*CP*AP*CP*TP*GP*GP*CP*CP*GP*TP*CP*GP*TP*TP*TP*CP*G)-3')	polymer	28	8591.5	4		Synthetic construct
3	I	DNA (5'-D(P*CP*GP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*(DOC))-3')	polymer	21	6411.2	1		Synthetic construct
4	A, B, C, D	DNA polymerase III subunit alpha	polymer	1220	137579.4	4	UniProt (Q9XDH5) Pfam (PF02811) Pfam (PF07733) Pfam (PF17657) Pfam (PF14579) In PDB	Thermus aquaticus
5	M, N, O, P	DNA polymerase III subunit gamma/tau	polymer	177	19600.3	4	UniProt (A0A0M9ACL9) Pfam (PF20964) In PDB	Thermus aquaticus
6	C, D, A, B	ZINC ION	non-polymer		65.4	12	Chemie (ZN)
7	C, D, A, B	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)

Sequence modifications

M, N, O, P: 373 - 540 (UniProt: A0A0M9ACL9)

PDB	External Database	Details
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
Lys 373	Glu 373	conflict
Met 541	-	expression tag
Pro 542	-	expression tag
Pro 543	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	687910.0
Non-Polymers*	Number of molecules	16
	Total formula weight	882.1
All*	Total formula weight	688792.1

*Water molecules are not included.