4IQJ
Structure of PolIIIalpha-Tauc-DNA complex suggests an atomic model of the replisome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 188.530, 94.970, 204.080 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.26631 |
| Rwork | 0.264 |
| R-free | 0.30474 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hpi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.272 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.370 |
| High resolution limit [Å] | 3.198 | 3.198 |
| Number of reflections | 112427 | |
| <I/σ(I)> | 11.8 | 1.8 |
| Completeness [%] | 93.8 | 93.8 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 298 | 0.1 M TRIS pH 8.8, 18% (w/v) polyethylene glycol 4000, 0.2 M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
