4IOF

Crystal structure analysis of Fab-bound human Insulin Degrading Enzyme (IDE)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Insulin-degrading enzyme	polymer	990	114561.6	2	UniProt (P14735) Pfam (PF00675) Pfam (PF05193) Pfam (PF16187) In PDB	Homo sapiens (human)	Abeta-degrading protease, Insulin protease, Insulinase, Insulysin
2	C, E	Fab-bound IDE, heavy chain	polymer	263	28201.7	2		Mus musculus
3	D, F	Fab-bound IDE, light chain	polymer	239	25982.1	2		Mus musculus
4	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5		water	water		18.0	1	Chemie (HOH)

Sequence modifications

A, B: 42 - 1019 (UniProt: P14735)

PDB	External Database	Details
Met 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
Ala 37	-	expression tag
Ala 38	-	expression tag
Gly 39	-	expression tag
Ile 40	-	expression tag
Pro 41	-	expression tag
Leu 110	Cys 110	conflict
Ser 171	Cys 171	conflict
Ala 178	Cys 178	conflict
Val 257	Cys 257	conflict
Leu 414	Cys 414	conflict
Asn 573	Cys 573	conflict
Ser 590	Cys 590	conflict
Ser 789	Cys 789	conflict
Ala 812	Cys 812	conflict
Ala 819	Cys 819	conflict
Ser 904	Cys 904	conflict
Asn 966	Cys 966	conflict
Ala 974	Cys 974	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	337490.7
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	337556.1

*Water molecules are not included.