4IMF
Crystal Structure of Pasteurella multocida N-Acetyl-D-Neuraminic acid lyase K164 mutant complexed with N-Acetylneuraminic acid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | N-acetylneuraminate lyase | polymer | 293 | 32575.4 | 2 | UniProt (Q9CKB0) Pfam (PF00701) In PDB | Pasteurella multocida subsp. gallicida | N-acetylneuraminate pyruvate-lyase, N-acetylneuraminic acid aldolase, Sialate lyase, Sialic acid aldolase, Sialic acid lyase |
| 2 | A, B | 5-(acetylamino)-3,5-dideoxy-D-glycero-D-galacto-non-2-ulosonic acid | non-polymer | 309.3 | 2 | Chemie (SI3) | |||
| 3 | A, B | 1-ETHOXY-2-(2-METHOXYETHOXY)ETHANE | non-polymer | 148.2 | 3 | Chemie (ME2) | |||
| 4 | A, B | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 5 | A | ISOPROPYL ALCOHOL | non-polymer | 60.1 | 2 | Chemie (IPA) | |||
| 6 | water | water | 18.0 | 253 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 293 (UniProt: Q9CKB0)
| PDB | External Database | Details |
|---|---|---|
| Ala 164 | Lys 164 | engineered mutation |
| Ser 278 | Pro 278 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 65150.8 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1289.7 | |
| All* | Total formula weight | 66440.5 |
