4IMF
Crystal Structure of Pasteurella multocida N-Acetyl-D-Neuraminic acid lyase K164 mutant complexed with N-Acetylneuraminic acid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | N-acetylneuraminate lyase | polymer | 293 | 32575.4 | 2 | UniProt (Q9CKB0) Pfam (PF00701) | Pasteurella multocida subsp. gallicida | N-acetylneuraminate pyruvate-lyase, N-acetylneuraminic acid aldolase, Sialate lyase, Sialic acid aldolase, Sialic acid lyase |
| 2 | C, I (A, B) | 5-(acetylamino)-3,5-dideoxy-D-glycero-D-galacto-non-2-ulosonic acid | non-polymer | 309.3 | 2 | Chemie (SI3) PubChem (349962) PubChem (51403833) PubChem (51403837) PubChem (51403839) PubChem (51403835) PubChem (59691305) PubChem (14017587) PubChem (91663215) PubChem (124922592) PubChem (124922593) PubChem (124922594) PubChem (129708853) PubChem (129708963) PubChem (135055133) | |||
| 3 | D, J, K (A, B) | 1-ETHOXY-2-(2-METHOXYETHOXY)ETHANE | non-polymer | 148.2 | 3 | Chemie (ME2) PubChem (13847) | |||
| 4 | E, F, L (A, B) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) PubChem (312) | |||
| 5 | G, H (A) | ISOPROPYL ALCOHOL | non-polymer | 60.1 | 2 | Chemie (IPA) | |||
| 6 | M, N (A, B) | water | water | 18.0 | 253 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 293 (UniProt: Q9CKB0)
| PDB | External Database | Details |
|---|---|---|
| Ala 164 | Lys 164 | engineered mutation |
| Ser 278 | Pro 278 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 65150.8 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1289.7 | |
| All* | Total formula weight | 66440.5 |
