4IM7

Crystal structure of fructuronate reductase (ydfI) from E. coli CFT073 (EFI TARGET EFI-506389) complexed with NADH and D-mannonate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Hypothetical oxidoreductase ydfI	polymer	506	55931.4	1	UniProt (Q8FHD0) Pfam (PF01232) Pfam (PF08125) UniProt (by SIFTS) (A0A0H2V7F2)	Escherichia coli
2	B (A)	D-MANNONIC ACID	non-polymer		196.2	1	Chemie (CS2)
3	C (A)	1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE	non-polymer		665.4	1	Chemie (NAI)
4	D (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	E (A)	water	water		18.0	471	Chemie (HOH)

Sequence modifications

A: 2 - 487 (UniProt: Q8FHD0)

PDB	External Database	Details
Met -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	55931.4
Non-Polymers*	Number of molecules	3
	Total formula weight	957.7
All*	Total formula weight	56889.1

*Water molecules are not included.