4IK3
High resolution structure of GCaMP3 at pH 8.5
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | RCaMP, Green fluorescent protein | polymer | 450 | 50544.4 | 1 | UniProt (K4DIE3) UniProt (P42212) Pfam (PF01353) Pfam (PF13499) In PDB | Entacmaea quadricolor (Bubble-tip anemone) | |
2 | A | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
3 | water | water | 18.0 | 240 | Chemie (HOH) |
Sequence modifications
A: 11 - 58 (UniProt: K4DIE3)
A: 61 - 150 (UniProt: P42212)
A: 159 - 301 (UniProt: P42212)
A: 302 - 450 (UniProt: K4DIE3)
PDB | External Database | Details |
---|---|---|
Met 1 | - | expression tag |
Gly 2 | - | expression tag |
Ser 3 | - | expression tag |
His 4 | - | expression tag |
His 5 | - | expression tag |
His 6 | - | expression tag |
His 7 | - | expression tag |
His 8 | - | expression tag |
His 9 | - | expression tag |
Gly 10 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Lys 65 | Met 153 | engineered mutation |
Ala 75 | Val 163 | engineered mutation |
Gly 87 | Ser 175 | engineered mutation |
Tyr 92 | Asp 180 | engineered mutation |
Val 115 | Thr 203 | engineered mutation |
Lys 118 | Ala 206 | engineered mutation |
Leu 143 | His 231 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 151 | - | linker |
Gly 152 | - | linker |
Thr 153 | - | linker |
Gly 154 | - | linker |
Gly 155 | - | linker |
Ser 156 | - | linker |
Met 157 | - | linker |
Val 158 | - | linker |
Leu 221 | Phe 64 | engineered mutation |
Cro 222 | Ser 65 | chromophore |
Cro 222 | Tyr 66 | chromophore |
Cro 222 | Gly 67 | chromophore |
Ile 250 | Val 93 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Leu 59 | - | linker |
Glu 60 | - | linker |
Thr 372 | Ile 354 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 50544.4 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 160.3 | |
All* | Total formula weight | 50704.7 |