4IK3
High resolution structure of GCaMP3 at pH 8.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-02-03 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.993, 46.614, 68.585 |
Unit cell angles | 90.00, 100.09, 90.00 |
Refinement procedure
Resolution | 31.011 - 2.007 |
R-factor | 0.1865 |
Rwork | 0.185 |
R-free | 0.21430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.966 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.011 | 2.040 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 25387 | |
Completeness [%] | 94.5 | 68.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | Tris pH 8.5, (NH4)2SO4, 23% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |