4IJX

Crystal structure of human Ap4A hydrolase E58A mutant complexed with DPO

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]	polymer	155	17864.3	2	UniProt (P50583) Pfam (PF00293) In PDB	Homo sapiens (human)	Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase, Ap4A hydrolase, Ap4Aase, Diadenosine tetraphosphatase, Nucleoside diphosphate-linked moiety X motif 2, Nudix motif 2
2	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
3	A	DIPHOSPHATE	non-polymer		173.9	1	Chemie (DPO)
4	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	B	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
6		water	water		18.0	121	Chemie (HOH)

Sequence modifications

A, B: 1 - 147 (UniProt: P50583)

PDB	External Database	Details
Ala 58	Glu 58	engineered mutation
Leu 148	-	expression tag
Glu 149	-	expression tag
His 150	-	expression tag
His 151	-	expression tag
His 152	-	expression tag
His 153	-	expression tag
His 154	-	expression tag
His 155	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	35728.7
Non-Polymers*	Number of molecules	5
	Total formula weight	480.3
All*	Total formula weight	36209.0

*Water molecules are not included.