4IJX
Crystal structure of human Ap4A hydrolase E58A mutant complexed with DPO
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical] | polymer | 155 | 17864.3 | 2 | UniProt (P50583) Pfam (PF00293) In PDB | Homo sapiens (human) | Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase, Ap4A hydrolase, Ap4Aase, Diadenosine tetraphosphatase, Nucleoside diphosphate-linked moiety X motif 2, Nudix motif 2 |
2 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
3 | A | DIPHOSPHATE | non-polymer | 173.9 | 1 | Chemie (DPO) | |||
4 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
5 | B | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
6 | water | water | 18.0 | 121 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 147 (UniProt: P50583)
PDB | External Database | Details |
---|---|---|
Ala 58 | Glu 58 | engineered mutation |
Leu 148 | - | expression tag |
Glu 149 | - | expression tag |
His 150 | - | expression tag |
His 151 | - | expression tag |
His 152 | - | expression tag |
His 153 | - | expression tag |
His 154 | - | expression tag |
His 155 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 35728.7 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 480.3 | |
All* | Total formula weight | 36209.0 |