4IIV

Crystal structure of a putative 3-oxoacyl-[acyl-carrier protein]reductase from Escherichia coli strain CFT073 complexed with NADP+ at 2.5 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	3-oxoacyl-[acyl-carrier protein] reductase	polymer	267	28461.4	4	UniProt (Q8FJ14) Pfam (PF13561) UniProt (by SIFTS) (A0A0H2V833)	Escherichia coli	beta-ketoacyl-acyl carrier protein reductase, FabG
2	E, F, G, H (A, B, C, D)	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	4	Chemie (NAP)
3	I, J, K, L (A, B, C, D)	water	water		18.0	299	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 243 (UniProt: Q8FJ14)

PDB	External Database	Details
Met -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag
Arg 20	Cys 20	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	113845.7
Non-Polymers*	Number of molecules	4
	Total formula weight	2973.6
All*	Total formula weight	116819.3

*Water molecules are not included.