4II3

Crystal structure of S. pombe Ubiquitin activating enzyme 1 (Uba1) in complex with ubiquitin and ATP/Mg

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Ubiquitin-activating enzyme E1 1	polymer	1001	111764.0	2	UniProt (O94609) Pfam (PF00899) Pfam (PF16190) Pfam (PF16191) Pfam (PF10585) Pfam (PF09358)	Schizosaccharomyces pombe (Fission yeast)	Poly(A)+ RNA transport protein 3
2	B, D (B, D)	Ubiquitin-60S ribosomal protein L40	polymer	96	10740.1	2	UniProt (P0CH07) Pfam (PF00240)	Schizosaccharomyces pombe (Fission yeast)
3	E, F, L, M (A, C)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	G, N (A, C)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
5	H, I, J, K, O... (A, C)	CALCIUM ION	non-polymer		40.1	10	Chemie (CA)
6	U, V, W, X (A, B, C, D)	water	water		18.0	149	Chemie (HOH)

Sequence modifications

A, C: 13 - 1012 (UniProt: O94609)

PDB	External Database	Details
Ser 12	-	expression tag

B, D: 1 - 76 (UniProt: P0CH07)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	245008.4
Non-Polymers*	Number of molecules	16
	Total formula weight	1512.4
All*	Total formula weight	246520.7

*Water molecules are not included.