4IFT

Crystal structure of double mutant thermostable NPPase from Geobacillus stearothermophilus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Thermostable NPPase	polymer	267	29684.8	2	UniProt (Q8L1N9) Pfam (PF13344) Pfam (PF13242) In PDB	Geobacillus stearothermophilus
2		water	water		18.0	223	Chemie (HOH)

Sequence modifications

A, B: 1 - 255 (UniProt: Q8L1N9)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Val 150	Ala 150	engineered mutation
Leu 157	Phe 157	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59369.6
All*	Total formula weight	59369.6

*Water molecules are not included.