4ID5

HIV-1 reverse transcriptase with bound fragment at the RNase H primer grip site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Reverse transcriptase/ribonuclease H	polymer	557	63989.2	1	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) In PDB	Human immunodeficiency virus type 1 (HIV-1)	EXORIBONUCLEASE H, P66 RT
2	B	P51 RT	polymer	429	50096.5	1	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) In PDB	Human immunodeficiency virus type 1 (HIV-1)	Gag-Pol polyprotein
3	A	4-{[4-({4-[(E)-2-cyanoethenyl]-2,6-dimethylphenyl}amino)pyrimidin-2-yl]amino}benzonitrile	non-polymer		366.4	1	Chemie (T27)
4	A, B	DIMETHYL SULFOXIDE	non-polymer		78.1	17	Chemie (DMS)
5	A	1-methyl-5-phenyl-1H-pyrazole-4-carboxylic acid	non-polymer		202.2	1	Chemie (1FF)
6	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7		water	water		18.0	889	Chemie (HOH)

Sequence modifications

A: 1 - 555 (UniProt: P03366)

PDB	External Database	Details
Met -1	-	expression tag
Val 0	-	expression tag
Ala 172	Lys 771	engineered mutation
Ala 173	Lys 772	engineered mutation
Ser 280	Cys 879	engineered mutation

B: 1 - 428 (UniProt: P03366)

PDB	External Database	Details
Gly 0	-	expression tag
Ser 280	Cys 879	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	114085.8
Non-Polymers*	Number of molecules	20
	Total formula weight	1921.2
All*	Total formula weight	116007.0

*Water molecules are not included.