4ID5
HIV-1 reverse transcriptase with bound fragment at the RNase H primer grip site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-07 |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.052, 72.872, 109.153 |
| Unit cell angles | 90.00, 100.47, 90.00 |
Refinement procedure
| Resolution | 29.201 - 1.950 |
| R-factor | 0.1879 |
| Rwork | 0.187 |
| R-free | 0.20890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.059 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.8_1069) |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 | 1.980 |
| High resolution limit [Å] | 1.950 | 2.150 | 1.950 |
| Rmerge | 0.064 | 0.232 | 0.553 |
| Number of reflections | 91214 | ||
| <I/σ(I)> | 20.3 | ||
| Completeness [%] | 99.7 | 100 | 100 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | pH 6.7 |
