4ID5

HIV-1 reverse transcriptase with bound fragment at the RNase H primer grip site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-07
Detector	ADSC QUANTUM 270
Spacegroup name	C 1 2 1
Unit cell lengths	162.052, 72.872, 109.153
Unit cell angles	90.00, 100.47, 90.00

Refinement procedure

Resolution	29.201 - 1.950
R-factor	0.1879
Rwork	0.187
R-free	0.20890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.059
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8_1069)
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	2.200	1.980
High resolution limit [Å]	1.950	2.150	1.950
Rmerge	0.064	0.232	0.553
Number of reflections	91214
<I/σ(I)>	20.3
Completeness [%]	99.7	100	100
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.7		pH 6.7