4ICK

Crystal structure of human AP4A hydrolase E58A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]	polymer	155	17864.3	2	UniProt (P50583) Pfam (PF00293)	Homo sapiens (human)	Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase, Ap4A hydrolase, Ap4Aase, Diadenosine tetraphosphatase, Nucleoside diphosphate-linked moiety X motif 2, Nudix motif 2
2	C (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3	D (B)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
4	E (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	F, G (A, B)	water	water		18.0	185	Chemie (HOH)

Sequence modifications

A, B: 1 - 147 (UniProt: P50583)

PDB	External Database	Details
Ala 58	Glu 58	engineered mutation
Leu 148	-	expression tag
Glu 149	-	expression tag
His 150	-	expression tag
His 151	-	expression tag
His 152	-	expression tag
His 153	-	expression tag
His 154	-	expression tag
His 155	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	35728.7
Non-Polymers*	Number of molecules	3
	Total formula weight	211.4
All*	Total formula weight	35940.1

*Water molecules are not included.