4IAO

Crystal structure of Sir2 C543S mutant in complex with SID domain of Sir4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	NAD-dependent histone deacetylase SIR2	polymer	492	55684.0	2	UniProt (P06700) Pfam (PF04574) Pfam (PF02146)	Saccharomyces cerevisiae	Regulatory protein SIR2, Silent information regulator 2
2	C, D (C, D)	Regulatory protein SIR4	polymer	159	18044.7	2	UniProt (P11978) Pfam (PF16991)	Saccharomyces cerevisiae (yeast)	Silent information regulator 4
3	E, G (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	F, H (A, B)	ADENOSINE-5-DIPHOSPHORIBOSE	non-polymer		559.3	2	Chemie (APR)
5	I, J, K, L (A, B, C, D)	water	water		18.0	110	Chemie (HOH)

Sequence modifications

A, B: 87 - 562 (UniProt: P06700)

PDB	External Database	Details
Met 71	-	expression tag
Gly 72	-	expression tag
Ser 73	-	expression tag
Ser 74	-	expression tag
His 75	-	expression tag
His 76	-	expression tag
His 77	-	expression tag
His 78	-	expression tag
His 79	-	expression tag
His 80	-	expression tag
Ser 81	-	expression tag
Gln 82	-	expression tag
Asp 83	-	expression tag
Pro 84	-	expression tag
Asn 85	-	expression tag
Ser 86	-	expression tag
Ser 543	Cys 543	engineered mutation

C, D: 737 - 893 (UniProt: P11978)

PDB	External Database	Details
Gly 735	-	expression tag
Ser 736	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	147457.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1249.5
All*	Total formula weight	148706.9

*Water molecules are not included.