4I7C

Siah1 mutant bound to synthetic peptide (ACE)KLRPV(23P)MVRPWVR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	E3 ubiquitin-protein ligase SIAH1	polymer	196	21978.1	2	UniProt (Q8IUQ4) Pfam (PF21361) Pfam (PF03145)	Homo sapiens (human)	Seven in absentia homolog 1, Siah-1, Siah-1a
2	B, D (B, D)	Protein phyllopod	polymer	14	1709.2	2	UniProt (Q27934) BIRD (PRD_000942)	Drosophila melanogaster
3	E, F, H, I (A, C)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
4	G, J (A, C)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	2	Chemie (MPD)
5	K, L, M (A, B, C)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

A, C: 90 - 282 (UniProt: Q8IUQ4)

PDB	External Database	Details
Gly 87	-	expression tag
Ser 88	-	expression tag
His 89	-	expression tag
Cys 156	Thr 156	engineered mutation

B, D: 113 - 125 (UniProt: Q27934)

PDB	External Database	Details
Ace 112	-	acetylation
Trp 123	Thr 123	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	47374.6
Non-Polymers*	Number of molecules	6
	Total formula weight	498.0
All*	Total formula weight	47872.6

*Water molecules are not included.