4I6L

Crystal structure of OTUB1 in complex with ubiquitin variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ubiquitin thioesterase OTUB1	polymer	229	26572.8	1	UniProt (Q96FW1) Pfam (PF10275) In PDB	Homo sapiens (human)	Deubiquitinating enzyme OTUB1, OTU domain-containing ubiquitin aldehyde-binding protein 1, Otubain-1, hOTU1, Ubiquitin-specific-processing protease OTUB1
2	B	Ubiquitin	polymer	76	8760.1	1	UniProt (P0CG48) Pfam (PF00240) In PDB	Homo sapiens (human)	Ubiquitin
3		water	water		18.0	84	Chemie (HOH)

Sequence modifications

A: 45 - 271 (UniProt: Q96FW1)

PDB	External Database	Details
Gly 43	-	expression tag
Ser 44	-	expression tag
Ser 91	Cys 91	engineered mutation

B: 1 - 74 (UniProt: P0CG48)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Lys 42	Arg 118	variant
Leu 44	Ile 120	variant
Arg 47	Gly 123	variant
His 62	Gln 138	variant
Phe 66	Thr 142	variant
Tyr 68	His 144	variant

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	35332.9
All*	Total formula weight	35332.9

*Water molecules are not included.