4I4T
Crystal structure of tubulin-RB3-TTL-Zampanolide complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Tubulin alpha-1B chain | polymer | 450 | 50041.3 | 2 | UniProt (P81947) Pfam (PF00091) Pfam (PF03953) | Bos taurus (bovine cow domestic cattle domestic cow) | Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain |
| 2 | B, D (B, D) | Tubulin beta-2B chain | polymer | 445 | 49999.9 | 2 | UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) | Bos taurus (bovine cow domestic cattle domestic cow) | |
| 3 | E (E) | Stathmin-4 | polymer | 143 | 16844.2 | 1 | UniProt (P63043) Pfam (PF00836) | Rattus norvegicus (brown rat rat rats) | Stathmin-like protein B3, RB3 |
| 4 | F (F) | Tubulin Tyrosine ligase, TTL | polymer | 384 | 44378.5 | 1 | UniProt (E1BQ43) | Gallus gallus (bantam chickens) | |
| 5 | G, V (A, C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 6 | BA, CA, H, P, W... (F, A, B, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) PubChem (888) | |||
| 7 | I, K, Q, X (A, B, C) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) PubChem (271) | |||
| 8 | J (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) PubChem (312) | |||
| 9 | EA, FA, L, M, N... (F, A, B) | GLYCEROL | non-polymer | 92.1 | 7 | Chemie (GOL) PubChem (753) | |||
| 10 | O, Y (B, D) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 11 | T (B) | TYROSINE | non-polymer | 181.2 | 1 | Chemie (TYR) PubChem (1153) PubChem (6057) PubChem (71098) | |||
| 12 | U (B) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) PubChem (4478249) | |||
| 13 | AA (D) | (2Z,4E)-N-[(S)-[(1S,2E,5S,8E,10Z,17S)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide | non-polymer | 497.6 | 1 | Chemie (ZPN) PubChem (70680599) PubChem (77909800) | |||
| 14 | DA (F) | PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER | non-polymer | 505.2 | 1 | Chemie (ACP) PubChem (4799) PubChem (46936826) PubChem (91532) PubChem (91885473) PubChem (134943439) PubChem (157749842) PubChem (157749839) | |||
| 15 | GA, HA, IA, JA, KA... (A, B, C, D, E...) | water | water | 18.0 | 1522 | Chemie (HOH) |
Sequence modifications
E: 3 - 145 (UniProt: P63043)
F: 1 - 378 (UniProt: E1BQ43)
| PDB | External Database | Details |
|---|---|---|
| Met 3 | Ile 47 | cloning artifact |
| Ala 4 | Ser 48 | cloning artifact |
| PDB | External Database | Details |
|---|---|---|
| His 379 | - | expression tag |
| His 380 | - | expression tag |
| His 381 | - | expression tag |
| His 382 | - | expression tag |
| His 383 | - | expression tag |
| His 384 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 261305.0 | |
| Non-Polymers* | Number of molecules | 26 |
| Total formula weight | 4298.3 | |
| All* | Total formula weight | 265603.3 |
