4I4T
Crystal structure of tubulin-RB3-TTL-Zampanolide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-01-29 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.99995 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.770, 158.640, 179.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.320 - 1.800 |
R-factor | 0.1728 |
Rwork | 0.171 |
R-free | 0.20430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ryc 3tin |
RMSD bond length | 0.008 |
RMSD bond angle | 1.179 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.400 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.103 | |
Number of reflections | 274535 | |
<I/σ(I)> | 13.35 | 1 |
Completeness [%] | 99 | |
Redundancy | 13.5 | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 3% PEG 4000, 4-6% glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 100 mM MES/Imidazole, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |