4I02

structure of the mutant Catabolite gene activator protein V140A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Catabolite gene activator	polymer	222	25049.9	6	UniProt (P0ACJ8) Pfam (PF00027) Pfam (PF13545) In PDB	Escherichia coli	cAMP receptor protein, cAMP regulatory protein
2	A, B, C, D, E...	ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE	non-polymer		329.2	9	Chemie (CMP)
3		water	water		18.0	927	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 210 (UniProt: P0ACJ8)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 140	Val 140	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	150299.5
Non-Polymers*	Number of molecules	9
	Total formula weight	2962.9
All*	Total formula weight	153262.4

*Water molecules are not included.