4I02
structure of the mutant Catabolite gene activator protein V140A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9687 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 116.260, 50.190, 276.322 |
| Unit cell angles | 90.00, 94.48, 90.00 |
Refinement procedure
| Resolution | 48.320 - 1.750 |
| R-factor | 0.18635 |
| Rwork | 0.185 |
| R-free | 0.21572 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.511 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 137.740 | 1.795 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 152519 | |
| Completeness [%] | 99.5 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 7-10% PEG3350, 15-20% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
