4HTF

Crystal structure of S-adenosylmethionine-dependent methyltransferase from Escherichia coli in complex with S-adenosylmethionine.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	S-adenosylmethionine-dependent methyltransferase	polymer	285	33162.1	1	UniProt (Q8XDG3) Pfam (PF13847)	Escherichia coli
2	B (B)	S-adenosylmethionine-dependent methyltransferase	polymer	285	33146.1	1	UniProt (Q8XDG3) Pfam (PF13847)	Escherichia coli
3	C, F (A, B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)
4	D, G (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	E (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
6	H (B)	BETA-MERCAPTOETHANOL	non-polymer		78.1	1	Chemie (BME)
7	I, J (A, B)	water	water		18.0	369	Chemie (HOH)

Sequence modifications

A: 1 - 261 (UniProt: Q8XDG3)

PDB	External Database	Details
Mse -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

B: 1 - 261 (UniProt: Q8XDG3)

PDB	External Database	Details
Mse -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66308.1
Non-Polymers*	Number of molecules	6
	Total formula weight	1126.2
All*	Total formula weight	67434.3

*Water molecules are not included.