4HN6

GR DNA Binding Domain R460D/D462R - TSLP nGRE Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	Glucocorticoid receptor	polymer	114	12759.8	2	UniProt (P04150) Pfam (PF00105)	Homo sapiens (human)	GR, Nuclear receptor subfamily 3 group C member 1
2	B (C)	DNA (5'-D(*CP*GP*CP*CP*TP*CP*CP*GP*GP*GP*AP*GP*AP*GP*CP*T)-3')	polymer	16	4900.2	1		synthetic construct
3	C (D)	DNA (5'-D(*AP*GP*CP*TP*CP*TP*CP*CP*CP*GP*GP*AP*GP*GP*CP*G)-3')	polymer	16	4900.2	1		synthetic construct
4	E, F, G, H (A, B)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	I, J, K, L (A, C, D, B)	water	water		18.0	43	Chemie (HOH)

Sequence modifications

A, B: 417 - 506 (UniProt: P04150)

PDB	External Database	Details
Met 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
Ser 400	-	expression tag
Ser 401	-	expression tag
Gly 402	-	expression tag
Val 403	-	expression tag
Asp 404	-	expression tag
Leu 405	-	expression tag
Gly 406	-	expression tag
Thr 407	-	expression tag
Glu 408	-	expression tag
Asn 409	-	expression tag
Leu 410	-	expression tag
Tyr 411	-	expression tag
Phe 412	-	expression tag
Gln 413	-	expression tag
Ser 414	-	expression tag
Asn 415	-	expression tag
Ala 416	-	expression tag
Asp 460	Arg 460	engineered mutation
Arg 462	Asp 462	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	35319.9
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	35581.6

*Water molecules are not included.