4HLU

Structure of the EcfA-A' heterodimer bound to ADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Putative ABC transporter ATP-binding protein TM_0222	polymer	268	30390.1	2	UniProt (Q9WY65) Pfam (PF00005)	Thermotoga maritima
2	B, D (D, C)	Energy-coupling factor transporter ATP-binding protein EcfA	polymer	268	29859.9	2	UniProt (Q9X1Z1) Pfam (PF00005)	Thermotoga maritima	ECF transporter A component EcfA
3	E, G, I, K (A, D, B, C)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)
4	F, H, J, L, M (A, D, B, C)	ACETATE ION	non-polymer		59.0	5	Chemie (ACT)
5	N, O, P, Q (A, D, B, C)	water	water		18.0	89	Chemie (HOH)

Sequence modifications

A, B: 2 - 266 (UniProt: Q9WY65)

PDB	External Database	Details
Gly -1	-	expression tag
Met 0	-	expression tag
Gly 1	-	expression tag

D, C: 1002 - 1259 (UniProt: Q9X1Z1)

PDB	External Database	Details
Gly 992	-	expression tag
Ser 993	-	expression tag
Gly 994	-	expression tag
Gly 995	-	expression tag
Ser 996	-	expression tag
His 997	-	expression tag
Met 998	-	expression tag
Gly 999	-	expression tag
Ser 1000	-	expression tag
Gly 1001	-	expression tag
Arg 1002	Lys 2	engineered mutation
Glu 1004	Thr 4	engineered mutation
Ala 1053	Glu 53	engineered mutation
Ala 1055	Glu 55	engineered mutation
Ala 1125	Glu 125	engineered mutation
Ala 1126	Lys 126	engineered mutation
Ala 1127	Glu 127	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	120500.1
Non-Polymers*	Number of molecules	9
	Total formula weight	2004.0
All*	Total formula weight	122504.1

*Water molecules are not included.