4HLU
Structure of the EcfA-A' heterodimer bound to ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2012-07-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | P 32 |
Unit cell lengths | 67.785, 67.785, 252.509 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.150 - 2.700 |
R-factor | 0.2155 |
Rwork | 0.213 |
R-free | 0.25830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yz2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.133 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.150 |
High resolution limit [Å] | 2.700 |
Number of reflections | 35999 |
<I/σ(I)> | 37.2 |
Completeness [%] | 99.9 |
Redundancy | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 100mM sodium acetate, 30% MPD, 200mM sodium chloride, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |