4HLU
Structure of the EcfA-A' heterodimer bound to ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2012-07-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 32 |
| Unit cell lengths | 67.785, 67.785, 252.509 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.150 - 2.700 |
| R-factor | 0.2155 |
| Rwork | 0.213 |
| R-free | 0.25830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yz2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.133 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.150 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 35999 |
| <I/σ(I)> | 37.2 |
| Completeness [%] | 99.9 |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 100mM sodium acetate, 30% MPD, 200mM sodium chloride, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
