4HG3

Structural insights into yeast Nit2: wild-type yeast Nit2 in complex with alpha-ketoglutarate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Probable hydrolase NIT2	polymer	341	38293.8	4	UniProt (P47016) Pfam (PF00795)	Saccharomyces cerevisiae (Baker's yeast)
2	BA, E, O, W (D, A, B, C)	2-OXOGLUTARIC ACID	non-polymer		146.1	4	Chemie (AKG)
3	CA, DA, F, G, H... (D, A, B, C)	GLYCEROL	non-polymer		92.1	16	Chemie (GOL)
4	AA, EA, M, N, U... (C, D, A, B)	CACODYLATE ION	non-polymer		137.0	7	Chemie (CAC)
5	FA, GA, HA, IA (A, B, C, D)	water	water		18.0	616	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 307 (UniProt: P47016)

PDB	External Database	Details
Met -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	153175.2
Non-Polymers*	Number of molecules	27
	Total formula weight	3016.8
All*	Total formula weight	156192.1

*Water molecules are not included.