Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4HG3

Structural insights into yeast Nit2: wild-type yeast Nit2 in complex with alpha-ketoglutarate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005575	cellular_component	cellular_component
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0016787	molecular_function	hydrolase activity
A	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
A	0043605	biological_process	amide catabolic process
A	0050406	molecular_function	[acetyl-CoA carboxylase]-phosphatase activity
A	0110050	molecular_function	deaminated glutathione amidase activity
B	0005575	cellular_component	cellular_component
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0016787	molecular_function	hydrolase activity
B	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
B	0043605	biological_process	amide catabolic process
B	0050406	molecular_function	[acetyl-CoA carboxylase]-phosphatase activity
B	0110050	molecular_function	deaminated glutathione amidase activity
C	0005575	cellular_component	cellular_component
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0016787	molecular_function	hydrolase activity
C	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
C	0043605	biological_process	amide catabolic process
C	0050406	molecular_function	[acetyl-CoA carboxylase]-phosphatase activity
C	0110050	molecular_function	deaminated glutathione amidase activity
D	0005575	cellular_component	cellular_component
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0016787	molecular_function	hydrolase activity
D	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
D	0043605	biological_process	amide catabolic process
D	0050406	molecular_function	[acetyl-CoA carboxylase]-phosphatase activity
D	0110050	molecular_function	deaminated glutathione amidase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE AKG A 401

Chain	Residue
A	LYS127
A	PHE131
A	CYS169
A	TYR170
A	ARG173
A	PHE195
A	THR196
A	THR199
A	HOH543

site_id	AC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 402

Chain	Residue
A	ARG58
A	TYR59
A	HOH525
A	HOH532
A	HOH641
B	ASP155
B	ILE156
B	HOH531

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 403

Chain	Residue
A	HIS117
A	PRO160
A	HOH542

site_id	AC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 404

Chain	Residue
A	ASP155
A	ILE156
A	HOH502
A	HOH561
A	HOH584
A	HOH627
B	ARG58
B	TYR59

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 405

Chain	Residue
A	ARG292
A	ASN293
A	PRO296
A	ASN299
A	HOH523
B	GLU98
B	LEU102

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL A 406

Chain	Residue
A	HOH639

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 407

Chain	Residue
A	ARG209
A	TRP262
A	GLY263
A	LYS264
A	HOH596
A	HOH631
C	ALA202

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 408

Chain	Residue
A	TYR59
A	LYS63
A	ASP232
A	GLU234
B	LYS151

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CAC A 409

Chain	Residue
A	ILE68
A	GLU124
A	HOH626
B	HIS240

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CAC A 410

Chain	Residue
A	GLU45
A	HIS93
A	GLU143
A	ARG250
A	HOH519
A	HOH597

site_id	BC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE AKG B 401

Chain	Residue
B	LYS127
B	PHE131
B	CYS169
B	TYR170
B	ARG173
B	ALA194
B	PHE195
B	THR196
B	THR199
B	HOH578

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL B 402

Chain	Residue
A	GLU98
B	ARG292
B	ASN293
B	PRO296
B	ASN299
B	HOH508

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL B 403

Chain	Residue
B	LYS36
B	LEU278
B	HOH567
C	GLU251
C	GOL403

site_id	BC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL B 404

Chain	Residue
A	GLU98
A	LEU101
B	LYS163
B	GLY185
B	ALA186
B	ARG292
B	HOH583

site_id	BC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL B 405

Chain	Residue
B	ARG209
B	TRP262
B	GLY263
B	LYS264
B	HOH522
B	HOH584
B	HOH604
D	ALA202
D	HIS203

site_id	BC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL B 406

Chain	Residue
C	LEU305
A	LYS151
B	TYR59
B	LYS63
B	ASP232

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CAC B 407

Chain	Residue
A	HIS240
B	LEU94
B	GLU124
B	HOH616

site_id	BC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CAC B 408

Chain	Residue
B	GLU45
B	LEU50
B	HIS93
B	GLU143
B	ARG250
B	HOH524
B	HOH589

site_id	CC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE AKG C 401

Chain	Residue
C	LYS127
C	PHE131
C	CYS169
C	TYR170
C	ARG173
C	ALA194
C	PHE195
C	THR196
C	THR199
C	HOH619

site_id	CC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL C 402

Chain	Residue
C	SER73
C	THR76
C	HOH538
C	HOH620

site_id	CC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL C 403

Chain	Residue
B	ARG7
B	LEU280
B	GOL403
C	CYS14
C	GLY226
C	MET227
C	HOH563

site_id	CC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CAC C 404

Chain	Residue
C	SER51
C	ASN53
C	SER146
C	ARG250
C	CAC405

site_id	CC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE CAC C 405

Chain	Residue
C	GLU45
C	LEU50
C	HIS93
C	ASN110
C	GLU143
C	VAL147
C	ARG250
C	CAC404
C	HOH657

site_id	CC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE AKG D 401

Chain	Residue
D	LYS127
D	PHE131
D	CYS169
D	TYR170
D	ARG173
D	ALA194
D	PHE195
D	THR196
D	THR199

site_id	CC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL D 402

Chain	Residue
B	ALA202
B	HIS203
D	ARG209
D	TRP262
D	GLY263
D	HOH548
D	HOH578

site_id	CC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL D 403

Chain	Residue
D	THR196
D	ILE197
D	LYS198
D	ARG249
D	ARG250
D	GLU251
D	HOH596

site_id	CC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CAC D 404

Chain	Residue
D	GLU45
D	HIS93
D	GLU143
D	VAL147
D	ARG250
D	HOH576
D	HOH598

Functional Information from PROSITE/UniProt

site_id	PS01227
Number of Residues	21
Details	UPF0012 Uncharacterized protein family UPF0012 signature. GsaICYDIrFPefslklrsmG

Chain	Residue	Details
A	GLY165-GLY185

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	837
Details	Domain: {"description":"CN hydrolase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00054","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00054","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	4
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00054","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	4
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PROSITE-ProRule","id":"PRU00054","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23897470","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23897470","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)