4HAZ
Crystal structure of CRM1 inhibitor Leptomycin B in complex with CRM1(R543S,K548E,K579Q)-Ran-RanBP1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | GTP-binding nuclear protein Ran | polymer | 216 | 24456.1 | 1 | UniProt (P62826) Pfam (PF00071) | Homo sapiens (human) | Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein |
| 2 | B (B) | Ran-specific GTPase-activating protein 1 | polymer | 140 | 16378.8 | 1 | UniProt (P41920) Pfam (PF00638) | Saccharomyces cerevisiae (yeast) | Chromosome stability protein 20, Perinuclear array-localized protein, Ran-binding protein 1, RANBP1 |
| 3 | C (C) | Exportin-1 | polymer | 1023 | 117327.6 | 1 | UniProt (P30822) Pfam (PF03810) Pfam (PF08389) Pfam (PF18777) Pfam (PF18784) Pfam (PF18787) Pfam (PF08767) | Saccharomyces cerevisiae (yeast) | CRM1, Chromosome region maintenance protein 1, Karyopherin-124 |
| 4 | D (A) | PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | non-polymer | 522.2 | 1 | Chemie (GNP) | |||
| 5 | E (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 6 | F, H, I, J, K (A, C) | CHLORIDE ION | non-polymer | 35.5 | 5 | Chemie (CL) PubChem (312) | |||
| 7 | G (C) | Leptomycin B | non-polymer | 540.7 | 1 | Chemie (LBF) PubChem (16760536) PubChem (435143) PubChem (44120032) PubChem (57459335) PubChem (58719437) PubChem (56984336) PubChem (5458861) PubChem (6436291) PubChem (73637) PubChem (6917907) PubChem (71684517) PubChem (73265316) PubChem (96327593) PubChem (96327594) PubChem (95065695) PubChem (95065694) PubChem (96327592) PubChem (125183499) PubChem (121232734) PubChem (123467862) PubChem (124023780) PubChem (125183497) PubChem (125183498) PubChem (133562712) PubChem (154730776) PubChem (162974035) PubChem (143594028) PubChem (162422456) PubChem (162974034) PubChem (162974036) PubChem (164817956) PubChem (175753429) | |||
| 8 | L, M, N (A, B, C) | water | water | 18.0 | 1175 | Chemie (HOH) |
Sequence modifications
B: 62 - 201 (UniProt: P41920)
C: 1 - 376 (UniProt: P30822)
C: 414 - 1058 (UniProt: P30822)
| PDB | External Database | Details |
|---|---|---|
| Lys 98 | Ala 98 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Cys 539 | Thr 539 | engineered mutation |
| Ser 543 | Arg 543 | engineered mutation |
| Glu 548 | Lys 548 | engineered mutation |
| Gln 579 | Lys 579 | engineered mutation |
| Cys 1022 | Tyr 1022 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 158162.5 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1264.5 | |
| All* | Total formula weight | 159427.0 |
